刘晓颖,阿力木·艾麦尔,樊栩,王为兰,王新绘,李金耀

• 1:新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
• 2:新疆大学资源与环境科学学院

摘要(Abstract)：

为探讨圣草酚对树突状细胞（dendritic cell, DC）成熟影响及其分子作用机制，基于网络药理学，通过多个数据平台获得圣草酚对DC、免疫抑制及炎症反应的相关靶点．采用Cytoscape 3.6.1软件构建活性成分潜在靶点网络，并利用String平台构建蛋白相互作用（PPI）网络．利用Metascape平台进行GO和KEGG富集分析，利用Autodock Vina软件进行分子对接．不同浓度（210μM、280μM和350μM）圣草酚单独或与细菌脂多糖（LPS）联用处理DC，流式细胞术检测细胞表面分子表达，ELISA检测细胞因子表达，Western blot检测基质金属蛋白MMP9的表达．获得圣草酚作用靶点96个，DC相关72个，免疫抑制相关14个，炎症反应相关48个，共有核心靶点包括AKT1、SRC、MMP9及MMP2等，主要关联氧化应激及细胞迁移等生物学过程，涉及癌症的信号通路、Ras信号通路、表皮生长因子受体酪氨酸激酶抑制剂抗性、c型凝集素受体信号通路、肿瘤坏死因子信号通路等信号通路．分子对接显示，圣草酚与MMP9具有更强的结合作用．体外实验显示，圣草酚能显著抑制LPS诱导的DC表面分子CD40(P<0.01)和CD86(P<0.001)、细胞因子TNF-α(P<0.001)及IL-6(P<0.001)以及基质金属蛋白酶MMP9(P<0.01)的表达．圣草酚通过抑制DC表面共刺激分子CD40和CD86、促炎细胞因子TNF-α和IL-6表达及MMP9等抑制DC成熟及迁移．

关键词(KeyWords)： 圣草酚;树突状细胞;网络药理学;免疫抑制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31760260）;; 新疆大学博士科研启动基金（BS160261）

作者(Author): 刘晓颖,阿力木·艾麦尔,樊栩,王为兰,王新绘,李金耀

DOI: 10.13568/j.cnki.651094.651316.2021.04.10.0002

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