王静,聂祥祥,李冠

• 1:新疆大学生命科学与技术学院

摘要(Abstract)：

以新疆主栽棉花品种新陆早19号和新陆早23号下胚轴为外植体,以GUS基因为报告基因,通过检测GUS基因在转化后的棉花下胚轴中瞬时表达情况,分析了菌液浓度、浸染时间、乙酰丁香酮浓度、超声波辅助处理、共培养温度和共培养时间等因素对GUS瞬时表达的影响.实验结果表明:菌液浓度OD600=0.5,浸染时间15 min,乙酰丁香酮浓度100μmol/L,24 C共培养72 h的转化条件下GUS基因瞬时表达率最高.

关键词(KeyWords)： 植物学;遗传转化;GUS瞬时表达;农杆菌;棉花

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30760030)项目资助

作者(Author): 王静,聂祥祥,李冠

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